[Hep-A-in-Vac, a Russian cultured concentrated inactivated vaccine against hepatitis A]
Gorbunov MA. Pavlova LI. Rozhkov SD. Kukharev AI. Novikov VIu. Karpovich LG. Kalashnikova TV. Bektimirov TA. Zelenov IA. Levina VD. Belashev BP. Ermolaeva TN. Doroshenko NV. Maidaniuk AT.
Tarasevich State Research Institute for Standartization and Control of Medical Biological Preparations, Moscow, Russia.
The results of the study of the reactogenicity, safety and immunological activity of Russian cultural vaccine against hepatitis A are presented. The vaccine was found to have specific safety, moderate reactogenicity and pronounced immunological activity. In addition, the study of the prophylactic efficiency of the vaccine in the pre-epidemic period of the outbreak of hepatitis A morbidity in a group exceeding 14,000 adults was carried out. The study revealed high prophylactic efficiency of the vaccine (its efficiency rate was equal to 97.7%). On the basis of materials thus obtained vaccine "Hep-A-in-Vac" was recommended for use in medical practice for the prophylaxis of hepatitis A among adults.
[The diagnosis of hepatitis C viral infection in blood donors and patients]
Ariamkina OL. Grigor'ev IuB. Fadeeva GE. Burganova RA. Maiorova GS. Khalilova RR. Fatullaeva EM. Arslanova RKh.
State University, Central Medico-Sanitary Institution, Ulyanovsk, Russia.
The data on the examination of 5139 serum samples, obtained from 3358 blood donors and 1781 somatic patients, for the presence of HCV, HBV and HDV markers. Antibodies (Ab) to HCV were detected, on the average, in 1.56% of the examined blood donors (207 persons). Of these, in 1994 HCV markers were detected in 1.98% and HBV markers in 6.74% of cases, while in 1995 these markers were detected in 1.14% and 3.43% of cases respectively. The diagnosis of chronic viral hepatitis (mainly C) was verified in 14% of seropositive donors. In the group of somatic patients Ab to HCV were diagnosed in 130 examined patients (7.3%), in a half of the cases HCV and HBV coinfection being present. Similar percentage of coinfection was detected in blood donors. It should be pointed out that out of the total number of somatic patients only 4 patients had chronic viral hepatic diseases. Our data were compared with the data of medical statistics and were found to reflect the spread of HCV and HBV infection in the region among different groups of the population.
[The characteristics of the caseinolytic activity of the contents of the large intestine in dysbiotic states]
Kuzikov AN. Bondarenko VM. Lykova EA. Stachenkova SV. Shakhmardanov MZ. Zemskova LN. Isaeva NP.
Gamaleya Research Institute of Epidemiology and Microbiology, Moscow, Russia.
In the course of the study of the biochemical rapid method for the evaluation of microecological changes in the intestine by means of the test for the caseinolytic activity of fecal supernatants, the simultaneous presence of several enzymes in the samples under test was shown. Different degrees of the thermal inactivation of the active principle, linked with the expression of the positive caseinolytic sample, were established. The manifestations of caseinolytic activity, differing in their thermal stability, were found to have the proteolysis zone of equal size. The study showed the stability of this sign and its preservation during 1.5-2 years of storage of fecal supernatants in a refrigerator. Some details of making tests according to this method and the conditions ensuring the standard reproducibility of results were ascertained.