[Molecular genetic and biochemical studies of human inherited diseases]
The paper gives the results of complex studies of human inherited diseases, which involved reverse genetics for the analysis of mutant genes, biochemical and immunological studies of anomalous proteins, the products of these genes. It describes the main results of the studies which used this complex approach to examining the etiology and pathogenesis of Wilson's disease, alpha 1-antitrypsin deficiency, familial hypercholesterolemia and cystic fibrosis.
[Experimental evidence for pharmacotherapy of cardiovascular and gastroduodenal diseases induced by the bodys extreme exposures]
Zavodskaia IS. Sapronov NS. Bul'on VV. Khnychenko LK.
The results of examining the mechanisms of development of neurogenic visceral abnormalities induced by the body's extreme exposures, search for modes of its pharmacological correction are presented. Experiments have provided evidence for that the neutropic agents recovering the functional activity of the sympathetic nervous system, normalizing energy and plastic exchange have a positive effect in the treatment of the cardiovascular and gastroduodenal diseases which are caused by a neurogenic agent. A basically new approach to treating peptic ulcer, coronary heart diseases, and myocardial infarction is proposed depending on the stage of a disease.
[Monoclonal antibodies in human hepatitis A virus immunoenzyme diagnosis]
Razumov IA. Tumanov IuV. Kazachinskaia EI. Tiunnikov GI. Loktev VB.
Antigen and antibody detection in EIA is a good tool in diagnosing HAV infections, especially in their differentiation from other hepatitides. Commercial kits containing polyclonal antibodies and murine MAbs to identify HAV are now available. Rat MAbs have not been assayed so far. Peroxidase-labelled rat MAbs and purified rat MAbs as antigen capture were used to modify commercial "VectoHep A-IgM" and "VectorHep A-Ag" kits. The results obtained with modified "VectoHep A-IgM" and "VectorHep A-Ag" kits with labelled rat MAbs suggest that labelled rat MAbs can increase the sensitivity and specificity of EIA. MAbs used as antigen capture and labelled antibodies permit at least an 8-fold increase in sensitivity as compared to polyclonal antibodies. The modified "VectorHep A-Ag" kit with labelled rat MAbs provided 100% sensitivity and specificity in EIA. The modified "VectorHep A-Ag" kit also allowed the authors to determine viral antigens in the cell lysate, homogenates of the infected monkey liver, stools from patients, and sewage water samples. The rat MAbs modified kits can be recommended for using in epidemiological and clinical studies of HAV infections.