[Flow cytometry sutdy of DNA and cell kinetics in the adenoma- carcinoma sequence in the large intestine]
Villanacci V. Grigolato PG. Cadei M. Cestari R. Ravelli P. Missale G.
Cattedra di Anatomia e Istologia Patologica, Universita degli Studi-Spedali Civili di Brescia.
The quantitation of DNA and growth fraction in the different step from dysplastic to neoplastic process in large bowel is the aim of this study. 70 colonic polyps were studied. The fresh specimens were processed and DNA analysis was carried out using a Partec CA II flow cytometer and the growth fraction was tested with KI-67 monoclonal antibody. The percentage of S-phase cells has been calculated with the Multicycle program. Our results demonstrated that 7 adenomas were tubulo-villous with mild dysplasia, 39 with mild-moderate dysplasia, 1 with severe dysplasia, 5 were polypoid carcinomas, 2 juvenile polyps, 1 polypoid leiomyoma, 1 inflammatory fibroid polyps. DNA analysis showed a diploid DNA content in non adenomatous polyps, in all adenomas with mild dysplasia, in 37 with mild-moderate dysplasia, in 8 cases with moderate-severe dysplasia and 1 cancer. Aneuploidy was discovered in 2 cases with mild-moderate dysplasia, in 6 cases with moderate-severe dysplasia, in the case of severe dysplasia and in 4 cases of carcinomas. Best indexes of linear correlation (Pearson's r) has been found between S-phase and DNA index (r = .75) and between S-phase and KI-67 (r = .82). IN CONCLUSION: 1) No relationship was found between DNA content and age, sex, size and location of polyps. 2) Aneuploidy is strictly related to moderate-severe grade of dysplasia therefore it is an important element in the development of adenomacarcinoma sequence. 3) DNA-index, S-phase and KI-67 are strictly related.
[Mixed mesodermal tumor of the ovary. Description of a case with a small primary neoplastic mass. Anatomo-clinical and histogenetic considerations]
Rinaldi P. Pasquini E. Papi M. Nicolini M. Tonelli U. Bernardi L.
Servizio di Anatomia Patologica e Citodiagnostica Ospedale Infermi di Rimini.
We describe a rare case of small mixed mesodermal tumor of the ovary (cm 1.5 in diameter) with widespread, clinically prevalent, peritoneal metastases. Some histological and immunohistochemical findings according with histogenetical conversion theory are discussed.
[Stromal tumor of the ileum (GIST) at the same time as a renal carcinoma. Description of a case and review of the literature]
Antonini C. Forgiarini O. Chiara A. Briani G. Belmonte P. Zucconelli R. Fiaccavento G. Sacchi G.
Servizio di Anatomia e Istologia Patologica, ULSS 10 Veneto Orientale San Dona di Piave, Venezia.
The gastrointestinal mesenchymal tumors from a heterogenous group that include several entities: leiomyomas, schwanomas and less differentiated tumors often referred as GIST. These neoplasm are uncommon and their clinical behaviour is most difficult to predict. We describe a malignant gastrointestinal stromal tumor of the ileum coexisting with renal cell carcinoma. The neoplasms were fixed in formaldehyde, embedded in paraffin and stained with hematoxylin-eosin. For immunohistochemical studies deparaffinized tissue sections were incubated with antibodies against vimentin, desmin, muscle specific actin, S100, CD34, GFAP, NSE and keratin. The epithelioid and spindle cells of ileal neoplasm were arranged in interlacing fascicle with occasional palisading and were positive for vimentin and CD34. Positivity for muscle specific actin was focally found. The renal neoplasm required differential diagnosis from metastatic GIST. The morphological and immunohistochemical investigations in our case were consistent with GIST coexisting with primitive renal cell carcinoma. One of the problems connected to the anatomo-clinical evaluation of GIST consist in the difficulty of making a prognosis. An almost complete review of the literature and view point on the topic has been performed. As a conclusion judging from papers regarding this argument, no clear parameters of biological behaviour exist excluding mitotic index.
[Case report of a double ano-rectal neoplasia (adenocarcinoma and melanoma)]
Marco AD. Baccini P. Pastorino A. Nozza P.
Istituto di Anatomia e Istologia Patologica, Unviersita di Genova.
The association in a single organ of two neoplasms with different, histogenensis is uncommon. A dual ano-rectal neoplasms, pigmented malignant melanoma in collision with adenocarcinoma is reported, along with a discussion concerning the problems related with primary origin of the melanocytic component.
[Neural infiltration in benign gallbladder lesions. Description of a case]
Cavazza A. Asioli S. Martella EM. De Marco L. Gardini G.
Servizio di Anatomia Patologica, Azienda Ospedaliera Arcispedale, S. Maria Nuova, Reggio Emilia.
Neural infiltration is considered a very useful marker of malignancy in the biliary tract. Aim of this study is to report a case of benign epithelium of the gallbladder with neural infiltration and to review the literature.